High throughput tissue distribution study of polymer-lipid nanoparticle formulations using DNA barcode technology

Aim: Our industry partner GeneStar Bioscience has synthesized a few novel pH-sensitive polymers that can be incorporated into lipid nanoparticles (LNPs) to enhance gene delivery. The preliminary data suggest that their polymers can change the structure of LNPs and redirect their tissue distribution after intravenous delivery. We hypothesize that polymer-lipid nanoparticles (pLNP) prepared with different pH-sensitive polymers will exhibit different tissue distribution profiles, and this project will be focused on testing this hypothesis.

Skills expected to develop: LNP and pLNP fabrication using microfluidics; LNP and pLNP characterization using dynamic light scattering, electrophoresis, and fluorescence spectrometry; animal study; DNA sequencing; oral presentation; manuscript writing

Impact: The project will provide proof-of-concept results on whether and how the novel pH-sensitive polymers change biodistribution of LNP. This will lead to the development of unique formulations for targeting different tissues to perform targeted gene therapy. An intern will receive cutting-edge training on non-viral gene delivery technology and gene therapy, eventually contributing to the sustainability and growth of the health sector of BC.